ophthalmologyОфтальмологияOphthalmology in Russia1816-50952500-0845Ophthalmology10.18008/1816-5095-2014-4-82-86ophthalmology-210Research ArticleКЛИНИЧЕСКИЕ И ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯCLINICAL & EXPERIMENTAL RESEARCHУльтраструктурные изменения хориоретинального комплекса при воздействии субпорогового лазерного излучения на длинах волн 0,81 и 0,532 мкм Экспериментальное исследованиеUltrastructural changes of chorioretinal complex under sub-threshold laser exposure at wavelengths of 0.81 and 0.532 μm (experimental study)ФедорукН. А.FedorukN. A.ФедоровА. А.FedorovA. A.sashvada@mail.ruБольшуновА. В.BolshunovA. V.ул.Россолимо, 11А, Москва, 119021, ФГ БУ «НИИ глазных болезней РАМН », Российская ФедерацияРоссияExperience Scientific-Research Institute of Eye Diseases of the Russian Academy of Medical Sciences, 11A, Rossolimo street, Moscow, 119021, Russian FederationRussian Federation2014140120151148286Copyright © Федорук Н.А., Федоров А.А., Большунов А.В., 20152015Федорук Н.А., Федоров А.А., Большунов А.В.Fedoruk N.A., Fedorov A.A., Bolshunov A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.ophthalmojournal.com/opht/article/view/210Цель

Цель: Выявление ультраструктурных изменений в хориоретинальном комплексе (ХРК) и возможности их обратимости при субпороговом воздействии лазерным излучением на длинах волн 532 мкм и 810 мкм через 1, 8 и 30 суток.

Методы

Методы: Экспериментальное исследование проводили на 32 глазах 16 кроликов породы серый шиншилла с использованием лазерного излучения на длинах волн 0.532 мкм и 0.81 мкм в непрерывном, микроимпульсном режимах, а также в режиме транспупиллярной термотерапии. Ультраструктурное исследование участков лазерного воздействия на ретинальную ткань выполняли с помощью трансмиссионного электронного микроскопа EM-10C («Opton», Германия).

Результаты

Результаты: Через 1 сутки после воздействия субпороговым лазерным излучением во всех исследуемых группах основные ультраструктурные изменения в ХРК происходили в слоях ретинального пигментного эпителия (РПЭ), хориокапиллярах (ХК) и фоторецепторах. Через 8 суток была отмечена внутриклеточная регенерация клеток РПЭ, а также частичное восстановление ХК кровотока и макрофагальной функции РПЭ. Через 30 суток после воздействия лазерным излучением практически полностью восстанавливалась морфология РПЭ, численность и структура наружных сегментов фоторецепторов на фоне регенерации митохондрий во внутренних сегментах.

Заключение

Заключение: Ультраструктурные повреждения ХРК после воздействия субпороговым лазерным излучением носят обратимый характер, и вследствие внутриклеточной регенерации к 30 суткам эксперимента происходит практически полное восстановление их клеточной структуры.

Aim: To reveal ultrastructural changes of chorioretinal complex and their possible reversibility under sub-threshold laser exposure at wavelengths of 0.81 and 0.532 μm in 1, 8 and 30 days. Methods: Experimental study was performed on 16 chinchilla rabbits (32 eyes) using laser at wavelengths of 0.532 and 0.81 μm in CW, micropulse, and transpupillary thermotherapy modes. Transmission electron microscope EM-10C (Opton, Oberkochen, Germany) was used to perform ultrastructural study of retina following laser exposure. Results: In a day after sub-threshold laser exposure, ultrastructural changes were mainly detected in retinal pigment epithelium (RPE), choriocapillaries, and photoreceptors. In 8 days, intracellular regeneration of RPE and partial restoration of choriocapillary blood flow and RPE macrophages were observed. In 30 days, RPE morphology, the number and the structure of photoreceptor outer segments were re-established. Mitochondria of photoreceptor inner segments regenerated as well. Conclusion: Ultrastructural changes of chorioretinal complex following sub-threshold laser radiation were reversible. Intracellular regeneration provided almost complete recovery of chorioretinal complex structure by day 30.

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The authors declare that there are no conflicts of interest present.