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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">ophthalmology</journal-id><journal-title-group><journal-title xml:lang="ru">Офтальмология</journal-title><trans-title-group xml:lang="en"><trans-title>Ophthalmology in Russia</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1816-5095</issn><issn pub-type="epub">2500-0845</issn><publisher><publisher-name>Ophthalmology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18008/1816-5095-2025-1-100-107</article-id><article-id custom-type="elpub" pub-id-type="custom">ophthalmology-2584</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>КЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>CLINICAL STUDIES</subject></subj-group></article-categories><title-group><article-title>Импрессионная цитология бульбарной конъюнктивы у пациентов рефракционного профиля</article-title><trans-title-group xml:lang="en"><trans-title>Impression Cytology of Bulbar Conjunctiva in Refractive Patients</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2519-8941</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дога</surname><given-names>А. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Doga</surname><given-names>A. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Дога Александр Викторович, доктор медицинских наук, профессор, заместитель генерального директора по научно‑клинической работе</p><p>Бескудниковский бульвар, 59а, Москва, 127486</p></bio><bio xml:lang="en"><p>Doga Aleksandr V., MD, Professor, deputy director</p><p>Beskudnikovsky blvd, 59A, Moscow, 127486</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9160-6240</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Борзенок</surname><given-names>С. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Borzenok</surname><given-names>S. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Борзенок Сергей Анатольевич, доктор медицинских наук, академик РАЕН, заведующий Центром фундаментальной офтальмологии</p><p>Бескудниковский бульвар, 59а, Москва, 127486</p></bio><bio xml:lang="en"><p>Borzenok Sergey A., MD, Academician of the Russian Academy of Sciences, Head of the Center for Fundamental Ophthalmology</p><p>Beskudnikovsky blvd, 59A, Moscow, 127486</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5601-8280</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мушкова</surname><given-names>И. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Mushkova</surname><given-names>I. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Мушкова Ирина Альфредовна, доктор медицинских наук, заведующая отделом лазерной рефракционной хирургии</p><p>Бескудниковский бульвар, 59а, Москва, 127486</p></bio><bio xml:lang="en"><p>Mushkova Irina A., MD, head of the Department of Laser Refractive Surger</p><p>Beskudnikovsky blvd, 59A, Moscow, 127486</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-6926-7780</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Каримова</surname><given-names>А. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Karimova</surname><given-names>A. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Каримова Аделя Насибуллаевна, кандидат медицинских наук, старший научный сотрудник отдела лазерной рефракционной хирургии</p><p>Бескудниковский бульвар, 59а, Москва, 127486</p></bio><bio xml:lang="en"><p>Karimova Adela N., PhD, Senior Researcher of the Department of Laser Refractive Surgery</p><p>Beskudnikovsky blvd, 59A, Moscow, 127486</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9933-9754</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Образцова</surname><given-names>М. Р.</given-names></name><name name-style="western" xml:lang="en"><surname>Obraztsova</surname><given-names>M. R.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Образцова Мария Романовна, аспирант</p><p>Бескудниковский бульвар, 59а, Москва, 127486</p></bio><bio xml:lang="en"><p>Obraztsova Maria R., postgraduate</p><p>Beskudnikovsky blvd, 59A, Moscow, 127486</p></bio><email xlink:type="simple">Obraztsova.Maria@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6378-8750</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Хубецова</surname><given-names>М. Х.</given-names></name><name name-style="western" xml:lang="en"><surname>Khubetsova</surname><given-names>M. Kh.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Хубецова Мадина Хетаговна, кандидат медицинских наук, заведующая глазным тканевым банком</p><p>Бескудниковский бульвар, 59а, Москва, 127486</p></bio><bio xml:lang="en"><p>Khubetsova Madina K., PhD, head of the eye tissue bank</p><p>Beskudnikovsky blvd, 59A, Moscow, 127486</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2817-7102</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Островский</surname><given-names>Д. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Ostrovskiy</surname><given-names>D. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Островский Дмитрий Сергеевич, кандидат медицинских наук, офтальмолог</p><p>Бескудниковский бульвар, 59а, Москва, 127486</p></bio><bio xml:lang="en"><p>Ostrovskiy Dmitriy S., PhD, ophthalmologist</p><p>Beskudnikovsky blvd, 59A, Moscow, 127486</p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">ФГАУ НМИЦ «МНТК “Микрохирургия глаза” им. академика С.Н. Федорова» Министерства здравоохранения Российской Федерации<country>Россия</country></aff><aff xml:lang="en">S.N. Fyodorov Eye Microsurgery Federal State Institution<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>30</day><month>03</month><year>2025</year></pub-date><volume>22</volume><issue>1</issue><fpage>100</fpage><lpage>107</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Дога А.В., Борзенок С.А., Мушкова И.А., Каримова А.Н., Образцова М.Р., Хубецова М.Х., Островский Д.С., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Дога А.В., Борзенок С.А., Мушкова И.А., Каримова А.Н., Образцова М.Р., Хубецова М.Х., Островский Д.С.</copyright-holder><copyright-holder xml:lang="en">Doga A.V., Borzenok S.A., Mushkova I.A., Karimova A.N., Obraztsova M.R., Khubetsova M.K., Ostrovskiy D.S.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.ophthalmojournal.com/opht/article/view/2584">https://www.ophthalmojournal.com/opht/article/view/2584</self-uri><abstract><p>Актуальность. Поддержание здоровой глазной поверхности очень важно как для выполнения качественной зрительной работы, так и для удовлетворительного социально активного образа жизни. Для защиты и поддержания нормального баланса глазной поверхности необходима стабильная и функциональная слезная пленка.Цель: определить плотность бокаловидных клеток бульбарной конъюнктивы у пациентов рефракционного профиля на до- и послеоперационном этапе с учетом состояния глазной поверхности.Пациенты и методы. В рамках данной работы были обследованы 62 пациента (62 глаза) рефракционного профиля, средний возраст которых составил 26,5 ± 2,1 года. Всем пациентам, помимо стандартной рефракционной диагностики, определяющей возможность безопасного проведения кераторефракционной хирургии, была проведена оценка статуса глазной поверхности: тест Ширмера-1, проба Норна, окрашивание глазной поверхности 0,1 % р-ром флюоресцеина и 0,1 % раствором лиссаминового зеленого, определение времени разрыва слезной пленки и потери секрета мейбомиевых желез при мейбографии верхнего и нижнего века на диагностическом модуле щелевой лампы Mediworks (Китай). Сроки наблюдения составили до операции, 1 неделя, 1, 3 и 6 месяцев после операции.Результаты. Было выявлено неравномерное распределение плотности бокаловидных клеток во всех группах исследования с последующим восстановлением к 6-му месяцу.Заключение. Результаты оценки состояния глазной поверхности с помощью стандартных офтальмологических методов исследования и с помощью современного диагностического модуля скрининга синдрома сухого глаза соответствовали нормальному течению восстановительного периода после кераторефракционной хирургии на фоне назначенной таргетной терапии в борьбе с индуцированным синдромом сухого глаза, в то время как исследование количества бокаловидных клеток показало наличие изменений, в частности, в группах с исходным наличием синдромом сухого глаза, что подтверждает высокую информативность и диагностическую значимость импрессионной цитологии.</p></abstract><trans-abstract xml:lang="en"><p>Actuality. Maintaining a healthy eye surface is very important both for performing high-quality visual work and for maintaining a socially active lifestyle. A stable and functional tear film is necessary to protect and maintain the normal balance of the eye surface.Purpose: to determine the density of goblet cells of the bulbar conjunctiva in refractive profile patients at the pre- and postoperative stages, taking into account the condition of the ocular surface.Patients and methods. Within the framework of this work, 62 (62 eyes) refractive profile patients were examined, their average age was 26.5 ± 2.1 years. In addition to the standard refractive diagnosis, which determines the possibility of safe keratorefractive surgery, all patients underwent an assessment of the status ocular surface: Schirmer-1 test, Norn test, staining of the ocular surface with 0.1% fluorescein and 0.1% lissamine green, determination of tear film break up time and loss of meibomian gland secretion during upper meibography and the lower eyelid on the diagnostic module of the Mediworks slit lamp (China). The follow-up periods were before surgery, 1 week, 1, 3 and 6 months after surgery.Results. The results of this study showed an uneven distribution of goblet cell density in all study groups, followed by recovery the 6th month.Conclusion. Thus, the results of assessing condition of the ocular surface using standard ophthalmological research methods and using a modern diagnostic module for screening dry eye syndrome corresponded to the normal course of the recovery period after keratorefractive surgery against the background of prescribed targeted therapy in the fight against induced dry eye syndrome, while a study of the number of goblet cells showed the presence of changes, in groups with the initial presence of dry eye syndrome, this confirms high informative value and diagnostic significance of impression cytology.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>бокаловидная клетка</kwd><kwd>слезная пленка</kwd><kwd>синдром сухого глаза</kwd><kwd>импрессионная цитология</kwd><kwd>кераторефракционная хирургия</kwd></kwd-group><kwd-group xml:lang="en"><kwd>goblet cell</kwd><kwd>tear film</kwd><kwd>dry eye syndrome</kwd><kwd>impression cytology</kwd><kwd>keratorefractive surgery</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Novack GD, Asbell P, Barabino S, Michael VW, Joseph B, Gary NF, Goldstein M, Michael AL, Schrader S, Stapleton F. 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