INFLUENCE OF NON-STEROIDAL ANTI-INFLAMMATORY EYE DROPS ON THE EPITHELIUM CELLS OF THE CORNEA AND CONJUNCTIVA IN VITRO

Objective: to evaluate the effect of NSAIDs eye drops with different preservatives on the viability of epithelial cells of human eyes in vitro. Materials and methods. The object of the study was four of the drug Broxinac®, Acular LS®, Diclo-F ®, Indocollyre®. As test-systems were used permanent cell lines of transformed conjunctival (Chang Conjunctiva, Clone 1-5c-4) and corneal (HCEC). The cytotoxicity of NSAID drops were assessed by the morphology and functional cells activity by the methods of phase contrast microscopy (PCM), MTT test and cell analysis in real time using the xCELLigence system. Results. It was found with MTT-test that cell lines HCEC are more sensitive to the cytotoxic action of the studied drugs than cell lines of Chang Conjunctiva, Clone 1-5C-4. The metabolic activity of conjunctiva cells in the presence of drugs, Acular LS® and Broxinac® was three times lower than in control; in the presence of drugs Indocollyre® and Diclo-F® — 20 times lower vs. the control. The metabolic activity of the cornea cells e in the presence of drugs, Acular LS® and Broxinac® was four times lower than in control; in the presence of drugs Indocollyre® and Diclo-F® viable cells were not identified. Drugs Indocollyre® and Diclo-F® has had a very high toxic effect on both cell types. The results of cell analysis xCELLIgence for both types of cells are consistent with the results of MTT test and in vivo observation of cells. Conclusion. Based on these data, there is the following gradation of NSAID eye drop’s cytotoxic potential: (reduce toxicity): Indocollyre® = Diclo-F® > Acular LS® = Broxinac®.

Cell cultures, xCELLigence, conjunctiva, preservatives, NSAID, cornea, cytotoxicity, ocular surface epithelium

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