New Properties of the Heparin-Containing Drug in vitro (Potential Antiviral and Anti-Inflammatory Effects)

Purpose: to study the cytological, immunological and antiviral effects of the HILOPARIN-KOMOD® drug in vitro.

Material and methods. We used transplantable cultures of normal cells of the human Chang conjunctiva, and the kidney cells of the Vero monkeys. The cytotoxic effect of the HILOPARIN-KOMOD® was  determined by the effect on the cell viability, and by optical density  (OP) of the monolayer of the Chang conjunctiva cell culture using the enzyme immunoassay (ELISA) with MTT. The effect of the drug on  the functional activity of conjunctival cells was evaluated by the  production of cytokines at the level of their in vitro transcription. The antiviral effect of the drug HILOPARIN-KOMOD® was studied on  the Vero cell line infected with herpes simplex virus type 1 (HSV-1) and HSV type 2 (HSV-2). The viral activity of HSV-1 and HSV-2  and the antiherpetic effect after the drug was evaluated by  polymerase chain reaction (PCR). 

Results. No significant cytotoxic effect on the metabolism of conjunctival cells of the preparation HILOPARINKOMOD ® in  dilutions from 1/2 to 1/2048 was revealed (in comparison with the  control). The effect of the drug in dilutions of 1/40 and 1/1000 on  the culture of the conjunctival cells resulted in suppression of the  production of interferon λ-1 mRNA (IFNλ-1) and IFNλ-2 mRNA  compared to the control. In the dilution of preparation 1/1000, the  production of mRNA of interleukin-6 (IL-6) was not revealed with  simultaneous presence of IL-10 mRNA. In different dilutions of the  drug, the number of copies of HSV-1 virus DNA decreased in  comparison with the control in all cases, the greatest antiviral effect  was achieved in a 1: 2 dilution. The effect of the preparation  HILOPARIN-KOMOD® on the culture of cells infected with HSV-2 in  dilutions of 1: 2 and 1: 5 led to a decrease in the level of viral replication by 300 and 40 times, respectively.

The conclusion. The drug HILOPARIN-KOMOD® has an anti-inflammatory effect that manifests itself in suppressing the synthesis of the mRNA of the acute phase proinflammatory cytokine IL-6 and  stimulating the production of anti-inflammatory IL-10 mRNA, without having a cytotoxic effect on conjunctival cells. The proven antiviral  effect of the drug makes it possible to recommend the inclusion of  HILOPARIN-KOMOD® in the scheme of therapy of patients with viral 
ophthalmopathology.

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