Mesenchymal stem cells with magnetic particles for the subretinal injection in ophthalmology
https://doi.org/10.18008/1816-5095-2013-3-72-74
Abstract
The technology of the cultivation of mesenchymal stem cells with magnetic particles for the subretinal injection was developed in this work. The estimation of the effect of magnetic particles on the proliferation and functional activity of mesenchymal stem cells has been done. We studied the induction of apoptosis and viability of stem cells with magnetic particles. The efficiency of the labeling of MSCs by magnetic particles for this technology is about 90 %, the viability of stem cells is about 95 %.
About the Authors
Yu. A. Beliy
Калужский филиал ФГБУ «МНТК «Микрохирургия глаза» им. акад. С. Н. Федорова» Минздрава России
Russian Federation
Russian Federation
A. A. Temnov
Научно-исследовательский институт скорой помощи им. Н. В. Склифосовского
Russian Federation
Russian Federation
A. V. Tereshchenko
Калужский филиал ФГБУ «МНТК «Микрохирургия глаза» им. акад. С. Н. Федорова» Минздрава России
Russian Federation
Russian Federation
S. A. Mirgorodskaya
ФГБУ «МНТК «Микрохирургия глаза» им. акад. С. Н. Федорова» Минздрава России
Russian Federation
Russian Federation
References
1. Сергеев В. С. Иммунологические свойства мультипотентных мезенхимальных стромальных клеток // Клеточная трансплантология и Тканевая Инженерия. — 2005. — № 2. — С. 39‑42.
2. Тахчиди Х. П., Гаврилова Н. А., Комова О. Ю. и др. Влияние стволовых / прогениторных клеток на функциональное состояние и степень выраженности дегенеративных изменений сетчатки у крыс линии Campbell // Офтальмохирургия. — 2010. — № 3. — С. 12‑15.
3. Aramant R., Seiler M. Progress in retinal sheet transplantation // Prog. Retin. Eye Res. — 2004. — V. 23, N 5. — P. 475‑494.
4. Arbab A., Jordan E., Wilson L. et al. In vivo trafficking and targeted delivery of magnetically labeled stem cells // Hum Gene Ther. — 2004. — Vol. 15, N. 4. —P. 351‑60.
5. Arragval S., Pittenger M. Human mesenchymal stem cells modulate allogeneic immune cell responses // Blood. — 2005. — V. 105. — P. 1815‑1822.
6. Bartholomew A., Sturgeon C., Siatskas M. et al. Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo //Exp. Hematol. — 2002. — V. 30. — P. 42‑48.
7. Kyrtatos P., Lehtolainen P., Junemann-Ramirez M. et al. Magnetic tagging increases delivery of circulating progenitors in vascular injury // JACC Cardiovasc Interv. — 2009. — Vol. 2. N. 8. — P. 794‑802.
8. Lazarus H., Koc O., Devine S. et al. Cotransplantation of HLA-identical sibling culture-expanded mezenchymal stem cells and hematopoetic stem cells in hematologic malignancy patients // Biol Blood Marrow Transplant. — 2005. — V. 5,
9. N. 11. — P. 389‑398.
10. Mimeault M., Batra S. Concise review: recent advances on the significance of stem cells in tissue regeneration and cancer therapies // Stem Cells. — 2006. — Vol. 24. — P. 2319‑2345.
11. Noort WA, Krusselbrink AB, in`t Anker PS et al. Mesenchymal stem cells promote engraftment of human umbilical cord blood-derived CD34 (+) cells in NOD / SCID mice // Exp Hematol — 2002. — V. 30. — P. 870‑878.
12. Sekiya I., Larson B., Smith J. et al. Expansion of human adult stem cells from bone narrow stroma: conditions that maximize the yields of early progenitors and evaluate their quality // Stem Cells. — 2002. — V. 20. — P. 530‑541.
13. Silverman M., Hughes S. Photoreceptor transplantation in inherited and environmentally induced retinal degeneration: anatomy, immunohistochemistry and function // Prog. Clin. Biol. Res. — 1989. — V. 314. — P. 687‑704.
14. Takahashi M., Palmer T., Takahashi J., Gage F. Widespread integration and survival of adult-derived neural progenitor cells in the developing optic retina // Mol Cell Neurosci. 1998. — V 12, N 6. — P.340‑348.
Review
For citations:
Beliy Yu.A., Temnov A.A., Tereshchenko A.V., Mirgorodskaya S.A. Mesenchymal stem cells with magnetic particles for the subretinal injection in ophthalmology. Ophthalmology in Russia. 2013;10(3):72-74. (In Russ.) https://doi.org/10.18008/1816-5095-2013-3-72-74
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