Preview

Ophthalmology in Russia

Advanced search

Lactoferrin Influence on the Course of Suppurative Corneal Ulcer

https://doi.org/10.18008/1816-5095-2023-1-128-135

Abstract

Research justification. Corneal ulcer is a common corneal pathology dangerous because of its complications, which occur in 2.5–37.9 % of cases despite modern treatment. Thus, the development of novel methods improving the results of corneal ulcer treatment is very important.

Research objective was to evaluate the efficacy of lactoferrin-based therapy for experimental suppurative corneal ulcer.

Material and methods. The study was performed in male chinchilla rabbits. Three intact rabbits (6 eyes) were used as control animals. Staphylococcus suppurative corneal ulcer was modeled in both eyes of 63 experimental animals. These rabbits were randomized into three groups. The first group was administered placebo therapy, the second group received antibacterial therapy, and the third group — instillations of lactoferrin. The area of ulcerative lesion was used as the measure of the therapeutic efficacy of the treatment. Then, a histological study was performed, and microsamples were photographed with LOMO TC-500 digital camera (Russia).

Results. Lactoferrin instillations resulted in reliable increase in the number of transfers from infiltration stage to epithelization stage, decrease in the percentage of ulcerations, and in the proportion of critical thinning of the cornea, as well as decrease in the proportion of perforations. However, it was noticed that lactoferrin treatment when continued after the end of epithelization process resulted in excessive formation of opaque scar tissue.

Conclusion. Local administration of lactoferrin stimulates the epithelium regeneration and the formation of the connective tissue and thus prevents the development of complications of suppurative corneal ulcer. However, the identified excessive formation of opaque scar tissue in lactoferrin treatment lasting too long has to be taken into consideration when developing the optimal scheme for lactoferrin treatment.

About the Authors

A. V. Kolesnikov
Ryazan State Medical University
Russian Federation

Kolesnikov Alexander V. - PhD, head of the Eye diseases department 

 Vysokovol’tnaya str., 9, Ryazan, 390026, Russian Federation 



E. R. Nemtsova
National Medical Radiology Research Center
Russian Federation

Nemtsova Elena R. - MD (Biol.), leading researcher, Department of experimental pharmacology and toxicology 

2nd Botkinskiy travel, 3, Moscow, 125284, Russian Federation 



M. M. Shishkin
National Medical and Surgical Center named after N.I. Pirogov
Russian Federation

 Shishkin Mikhail M. - MD, Professor, head of the Eye diseases department 

Nizhnyaya Pervomayskaya str., 70, Moscow, 105203, Russian Federation 



A. V. Shchul’kin
Ryazan State Medical University
Russian Federation

Shchul’kin Alexey V. - MD, Associate Professor, Professor of the Department of pharmacology with a pharmacy course 

Vysokovol’tnaya str., 9, Ryazan, 390026, Russian Federation 



O. I. Barenina
Ryazan State Medical University
Russian Federation

Barenina Olga I. - postgraduate of the Eye diseases department 

 Vysokovol’tnaya str., 9, Ryazan, 390026, Russian Federation 



I. V. Kirsanova
Ryazan State Medical University
Russian Federation

Kirsanova Irina V. - postgraduate of the Eye diseases department 

Vysokovol’tnaya str., 9, Ryazan, 390026, Russian Federation 



References

1. Kasparova E.A. Purulent corneal ulcers: etiology, pathogenesis. Annals of Ophthalmology = Vestnik oftal’mologii. 2015;5:87–97 (In Russ.). DOI: 10.17116/oftalma2015131587-97

2. Drozdova E.A., Maracheva N.M., Pukhova O.V., Timoshevskaya E.I. Bacterial corneal diseases: characteristic features and outcomes. Bashkortostan Medical Journal = Meditsinskiy vestnik Bashkortostan. 2017;12.2(68):29–32 (In Russ.).

3. Austin A, Lietman T, Rose-Nussbaumer J. Update of the management of infectious keratitis. Ophthalmology. 2017;124(11):1678–1689. DOI: 10.1016/j.ophtha.2017.05.012

4. Vogel HJ. Lactoferrin, a bird’s eye view. Biochem Cell Biol. 2012;90:233–244. DOI: 10.1139/O2012-016

5. Bruni N, Capucchio MT, Biasibetti E, Pessione E, Cirrincione S, Giraudo L, Corona A, Dosio F. Antimicrobial activity of Lactoferrin related peptides and applications in human and veterinary medicine. Molecules. 2016;21:752–777. DOI: 10.3390/molecules21060752

6. Kruzel M., Zimecki M., Actor J.K. Lactoferrin in a Context of Inflammation-Induced Pathology. Frontiers in Immunology. 2017;8:1438. DOI: 10.3389/fimmu.2017.01438

7. Fine D.H., Toruner G.A., Velliyagounder K.A Lactotransferrin single nucleotide polymorphism demonstrates biological activity that can reduce susceptibility to caries. Infection and Immunity. 2013;81:1596–605. DOI: 10.1128/IAI.01063-12

8. Legrand D. Overview of Lactoferrin as a natural immune modulator. J Pediatr. 2016;173 Suppl:S10–15. DOI: 10.1016/j.jpeds.2016.02.071

9. Adamova N.A., Gorgiladze T.U., Artemov A.V. The effectiveness of the treatment of corneal ulcers with fibronectin. Russian ophthalmological journal = Rossiyskiy oftal’mogicheskiy zhurnal. 1990;1:245–248 (In Russ.).

10. Ting D.S.J., Ho Ch. Sh., Deshmukh R., Said D.G., Dua H.S. Infectious keratitis: an update on epidemiology, causative microorganisms, risk factors, and antimicrobial resistance. Eye. 2021;35:1084–1101.

11. Teweldemedhin M., Gebreyesus H., Atsbaha A.H., Asgedom S.W., Saravanan M. Bacterial profile of ocular infections: a systematic review. BMC Ophthalmology. 2017;17(1):212. DOI: 10.1186/s1288-6017-0612-2

12. Das S., Samantaray R., Mallick A., Sahu S.K., Sharma S. Types of organisms and in vitro susceptibility of bacterial isolates from patients with microbial keratitis: A trend analysis of 8 years. Indian Journal of Ophthalmology. 2019;67(1):49–53. DOI: 10.4103/ijo.IJO_500_18

13. Yani E.V., Golikova V.A. Features of anti inflammatory therapy of bacterial corneal ulcers. Annals of Ophthalmology = Vestnik oftal’mologii. 2020;136(6):207–212 (In Russ.). DOI: 10.17116/oftama2020136062207

14. Shaimova V.A. Clinical and etiological features of various types of purulent corneal ulcers. Annals of Ophthalmology = Vestnik oftal’mologii. 2002;118(1):39–41 (On Russ.).

15. Farnaud S., Evans R.W. Lactoferrin — a multifunctional protein with antimicrobial properties. Molecular Immunology. 2003;40(7):395–405. DOI: 10.1016/s0161-5890(03)00152-4

16. Grammatikova N.E., Nemtsova E.R., Yakubovsky R.I. In Vitro Study of Antimicrobial Activity of Lactoferrins from Various Sources. Drug development & registration = Razrabotka i registraciâ lekarstvennyh sredstv. 2015;1(10):152–158 (In Russ.).

17. Borzenkova N.V., Balabushevich N.G., Larionova N.I. Lactoferrin: physicochemical properties, biological functions, delivery systems, drugs and biologically active additives (review). Russian Journal of Biopharmaceuticals = Biofarmacevticheskiy zhurnal. 2010;2(3):3–19 (In Russ.).

18. Gutteridge J.M.C., Paterson S.K., Segal A.W., Halliwell B. Inhibition of lipid peroxidation by the iron binding protein lactoferrin. Biochemical Journal.1981;199:259–261.

19. Osipova N.A., Edeleva N.V., Yakubovskaya R.I., Nemtsova Ye.R., Chissov V.I. Oxidative Stress in Critical Conditions and its Correction. General Reanimatology = Obshhaja reanimatologija. 2008;4(2):98–102 (In Russ.).

20. Chissov V.I., Yakubovskaya R.I., Nemtsova E.R., Osipova N.A., Edeleva N.V., Utkin M.M., Zvyagin A.A. Antioxidants treatment of severe post operative pyoinflammatory and septic complications. Surgery. Journal named N.I. Pirogov = Khirurgiya. Zhurnal imeni N.I. Pirogova. 2008;11:14–19 (In Russ.).

21. Takayama Y., Takezawa T. Lactoferrin promotes collagen gel contractile activity of fibroblasts mediated by lipoprotein receptors. The International Journal of Biochemistry and Cell Biology 2006;84(3):268–274. DOI: 10.1139/o06-041


Review

For citations:


Kolesnikov A.V., Nemtsova E.R., Shishkin M.M., Shchul’kin A.V., Barenina O.I., Kirsanova I.V. Lactoferrin Influence on the Course of Suppurative Corneal Ulcer. Ophthalmology in Russia. 2023;20(1):128-135. (In Russ.) https://doi.org/10.18008/1816-5095-2023-1-128-135

Views: 437


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 1816-5095 (Print)
ISSN 2500-0845 (Online)