Experimental study of VEGF immune expression dynamics in the retina using photoinduced BRVO model
https://doi.org/10.18008/1816-5095-2014-2-32-38
Abstract
Aim. To describe the dynamics of vascular endothelial growth factor (VEGF) immune expression in the retina using the model of photoinduced branch retinal vein occlusion (BRVO) and to establish the terms of neovascularization appearance.
Materials and methods. BRVO was modelled on 21 chinchilla rabbits (21 eyes) weighing 1.5‑2 kg (fellow eyes served as controls). Photosensitizer «Fotoditazin» (2.5 mg / kg) was injected intravenously. 15 min later, transpupillary laser irradiation of branch retinal vein near the optic nerve head was performed. Irradiation energy density was 200 J / cm2. Histological analysis and immunohistochemistry of the retina was performed following 30 min, at days 1, 2, 3, 7, 14 and 30.
Results. Maximum VEGF accumulation in photoinduced BRVO model was observed on day 2. From day 3, direct neovascularization was confirmed. VEGF levels were stably high throughout the follow-up to the day 30 inclusive.
Conclusion. VEGF immune expression in the retina using the model of BRVO induced by photodynamic exposure was explored for the first time. These data can serve as the basis for future studies in order to define optimal anti-VEGF agent, its dosage and terms to manage this condition.
About the Authors
Yu. A. Belyy
Kaluga branch of Microsurgery institution
Russian Federation
Russian Federation
A. V. Tereshchenko
Kaluga branch of Microsurgery institution
Russian Federation
Russian Federation
A. V. Shatskikh
Microsurgery institution
Russian Federation
Russian Federation
D. S. Velibekova
Kaluga branch of Microsurgery institution
Russian Federation
Russian Federation
A. V. Revishchin
Institute of gene Biology
Russian Federation
Russian Federation
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Review
For citations:
Belyy Yu.A., Tereshchenko A.V., Shatskikh A.V., Velibekova D.S., Revishchin A.V. Experimental study of VEGF immune expression dynamics in the retina using photoinduced BRVO model. Ophthalmology in Russia. 2014;11(2):32-38. (In Russ.) https://doi.org/10.18008/1816-5095-2014-2-32-38
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